MICRO FLAME PHOTOMETRIC DETERMINATION OF SODIUM, POTASSIUM, AND CALCIUM IN SERUM WITH ORGANIC SOLVENTS
نویسندگان
چکیده
منابع مشابه
Simultaneous determination of lithium, sodium and potassium in blood serum by flame photometric flow-injection analysis.
A simultaneous flow-injection analysis (FIA) manifold that could analyse three ions from a single injection was designed, constructed, calibrated and used successfully to analyse Li(+), Na(+) and K(+). This FIA method was 10 times faster than the batch technique. The sample volume required was a fraction of about 1/110 to 1/75 that of the batch technique. The outputs were quite reproducible and...
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Separated erythrocytes are washed repeatedly with iso-osmolar magnesium chloride solution, lysed by adding saponin, and sodium potassium measured in the diluted hemolysate by flame photometry. The coefficient of variation for the method was less than 4%. Reference intervals determined for a healthy population and hospitalized (elective surgery) patients without electrolyte disorders were 4.6-7....
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The convenience and accuracy of flame photometric methods for the determination of sodium and potassium in body tissues and fluids suggest the desirability of determining other cations such as calcium and magnesium in the same sample. While reliable procedures for the determination of sodium and potassium have been developed (1, 2), there are apparently no satisfactory flame spectrophotometric ...
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Serum sodium and potassium analyses by continuous-flow flame photometry is a well-accepted procedure (1, 2). Recently Habig and Williamson (3) have used the widely available Instrumentation Laboratory Model 143 (IL-143) flame photometer for continuousflow analyses. Pennacchia et al. (4) have operated this flame photometer at rates as high as 120 samples per hour. In both systems the results app...
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Powell (1953) introduced a method for the estimation of serum calcium using the EEL flame photometer, and claimed that it gave results in good agreement with a standard titrimetric procedure. In this laboratory, both these methods were found to give low results when tested against standard sera, prepared by adding known amounts of calcium to serum completely decalcified by treatment with a cati...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)50852-3